Application

Western blot: 1 µg/ml for 2 hours at RT (1:200 dilution)

Immunofluorescence

Immunohistochemistry (frozen and paraffin): 2-4 µg/ml for 30 minutes at RT

(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling for 20 min. at RT)

Optimal working dilutions must be determined by end user.

Research CategoryApoptosis & Cancer

Research Sub CategoryBCL2 & Inhibition

Anti-Bax Antibody, N-terminus, aa3-16 hBax protein, clone 2D2 is an antibody against Bax for use in IF, WB, IH(P).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Synthetic peptide corresponding to aa 3-16 (GSGEQPRGGGPTSS) of human bax. This amino acid sequence is not shared by mouse or rat Bax.

Epitope: N-terminus, aa3-16 hBax protein

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-434

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.

Specificity

Recognizes a 21 kD protein. Does not cross-react with Bcl-2a or Bcl-X. In contrast to Bcl-2a and Bcl-X, overexpression of Bax protein accelerates apoptosis.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.